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This Article describes the biology of Agrobacterium tumefaciens and a deeper insight into the mechanism of T-DNA transfer with special reference to vector design in recombinant DNA technology. A brief description of various types of opines and chemo attraction of bacteria, crown gall disease is also provided. A number of practice MCQ related to national level fellowship exams are also provided for the students.
This Article describes the fundamentals of mole concept in a simplified manner with numerous examples and analogies. Although the article is written keeping in view the problems of life science students, however school students and teachers may also potentially make use of this article in refining the understanding. Recent updates on mole concept, experimental evidences for the determination of Avogadro's number have also been incorporated.
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